PECULIARITIES OF METABOLISM OF GLYCOCONJUGATES IN MONITORING OF OVARIAN CANCER TREATMENT
M.Knyazyeva (1), A.Prokopyuk (2), O.Babaeva (3)
(1) Department of Biochemistry, Kharkov National University named after V.N.Karazin, Kharkov, Ukraine
(2) Department of Oncogynaecology, Civil Organization \"New thinking in medicine\", Kharkov, Ukraine
(3) Department of Biochemistry, Civil Organization \"New thinking in medicine\", Kharkov, Ukraine
Contact E-mail: marina_k@tnss.kharkov.ua
In order to define optimal number of neoadjuvant chemotherapy (NChT)-courses and to improve the treatment efficiency of patients having ovarian cancer (OC) we determined summary content of glycosaminoglycans (GAG) and their fractional composition as well as summary content of chondroitinsulfates (Ch-S) in the blood serum. 47 women having OC of III-IV stages before and after NChT in dynamics and 12 women having benign tumor (BT) have been examined. Platinum preparations in the combination with cyclophosphan and doxorubicine were used for NChT (1-6 courses). The obtained results show that before NChT summary content of GAG in the blood serum of patients having OC was higher than summary content of GAG in the patients having BT as well as GAG fraction I contained mainly Ch-6-S and fraction II contained Ch-4-S and dermatansulfate. The first course of NChT led to the reduction of summary content of GAG to the GAG level in patients having BT. It also led to the reduction of GAG fractions II and III (contained heparansulfates, keratansulfates and heparin), but at the same time the content of Ch-6-S increased as compared with the GAG level in patients having BT and OC without NChT. Structural degradation of matrix effects the GAG spectrum and precedes invasion and tumor metastasis. Great number of NChT courses (6) modules neoplastic activity best of all and prevents invasion and metastasis that effects the GAG spectrum in the blood serum of patients.
Marks:
GAGs-sulfated glycosaminoglycans - NChT - neoadjuvant chemotherapy (1-6 courses)
Ch-S - chondroitinsulfates
D-S - dermatansulfate
H-S - heparansulfates
K-S - keratansulfates
H - heparin
BT -benign tumor
OC- ovarian cancer
F1- first fraction of GAGs contains Ch-6-S
F2- second fraction of GAGs contains Ch-4-S and D-S
F3- third fraction of GAGs contains H, H-S, K-S
Coefficients:
K1 = ∑ GAGs / F1
K2 = ∑ GAGs / F2
K3 = ∑ GAGs / F3
K4 = (F2+F3) / F1
100%- levels of GAGs, F1, F2, F3,K1, K2, K3, K4 of healthy persons
